From campanellj from mail.montclair.edu Tue Jul 1 18:34:50 2008 From: campanellj from mail.montclair.edu (James J. Campanella) Date: Wed Jul 2 12:55:50 2008 Subject: [Plant-biology] pCambia1201 question Message-ID: Dear fellow workers in the mines of science, I have been tryingto work with the pCambia1201 plasmid for the lastseveral weeks, but ithas been driving my lab a bit crazy. My problemis that it does not wantto digest with most endonucleases we've tried.I simply can not use theplasmid if I can't cut it, so I have aproblem. It was suggested to meto try transferring the plasmid to DH5cells, which I did to no avail--the resultant plasmid still does notcut with almost every enzyme in mylibrary. I was finally able to getit to cut with XhoI and PvuII, buteven those were only partialdigests. Does anybody have anyadvice on working with this thing? The literaturedoes not suggest thatthere is even a problem, and the group thatengineered it could onlysuggest "trying all your enzymes until youfind one that cuts", which isa bit impractical. Thanks for any help, Jim Campanella ------------------- James J. Campanella, Associate Professor, Department of Biology and Molecular Biology Montclair State University 1 Normal Avenue Montclair, NJ 07043 Alternate email address: jcamp@alumni.uchicago.edu Ph: 973-655-4097 Fax: 973-655-7047 From CFLANAGIN from tx.rr.com Fri Jul 11 21:43:27 2008 From: CFLANAGIN from tx.rr.com (Crystal Flanagin) Date: Sat Jul 12 11:54:29 2008 Subject: [Plant-biology] What kind of house plant is this? Message-ID: <86ECAC9260B749DCBAAE55761A3ED184@ownerPC> My father in law gave me a plant almost a year ago and I cannot find any one that can identify it. When I got it he had it in a small plastic bottle with water, it looked unkempt so I cleared the dead branches and planted it in soil in a small pot. It didn't seem to like much sun as it would kind of wither up. It has thin fuzzy stalks with small oblong leaves. Every few weeks the entire outside of it turns brown and sheds like a snake. I thought it was dying the first time it happened but it seems to be getting longer in the stalk, and turns light green when the outside sheds off. I am down to just a few stalks and would like to either figure out a way to reroot this one or buy more. Please help, I don't have any pictures but could take some to show you if you need me to. From parthagen from gmail.com Sun Jul 13 07:53:27 2008 From: parthagen from gmail.com (Parthagen) Date: Sun Jul 13 13:55:15 2008 Subject: [Plant-biology] Re: Weedkiller, Nettles and any danger. References: Message-ID: <00c33286-ce1b-4536-a018-c70d62c0af68@34g2000hsf.googlegroups.com> hai J Wick, can you give the ame and composition of the weed killer? may be that can help to track the problem. parthagen On Jul 4, 8:23?pm, JWick wrote: > Recently my Mum has sprayed the garden with a certain weedkiller that > gets to the roots. After a while as I was walking in the garden with > bare feet my left foot was brushed by the nettles and got stung. > However, the pain lasted throughout the day and into the night, whereas > normally the pain wouldn't last for a minute. I was concerned whether > this continued pain was due to the weedkiller, and whether the poison > could have absorbed it and entered my body. > > Would this be valid? Should I take any further action? > > Thanks > > -- > JWick From harryi from sembiosys.com Mon Jul 14 10:33:33 2008 From: harryi from sembiosys.com (Indra Harry) Date: Mon Jul 14 11:27:00 2008 Subject: [Plant-biology] Gel Strength Message-ID: I would like info on low cost instruments used for measuring media gel strengths. Thanks, Indra ________________________________ Indra S. Harry, Ph.D Group Leader, Safflower Transformation SemBioSys Genetics Inc. 110, 2985-23rd Avenue NE Calgary, Alberta, Canada T1Y 7L3 Phone: (403) 250- 5424 ext. 784 Direct Line: (403) 717- 8784 Fax: (403) 250 - 3886 Email: harryi@sembiosys.com _________________________________ CONFIDENTIALITY NOTICE: This communication, including any attachments, may contain information that is confidential and/or privileged and is intended for the exclusive use of the individual to whom it is addressed (the "addressee"). Any review, distribution, copying, disclosure or release of the information contained within this communication by a person other than the addressee is strictly prohibited. If you have received this communication in error, please notify the sender immediately and delete all copies of this message, including any attachments. From ramon from bisque.com Wed Jul 16 11:46:01 2008 From: ramon from bisque.com (Ramon E. Bisque) Date: Wed Jul 16 13:16:36 2008 Subject: [Plant-biology] Direction of vine winding Message-ID: <0B1BB17697572B4F9F0F40ACFC5BB3DA013F8D44@draco.bisque.com> Began searching when I noticed that my pole beans were all climbing clockwise and a bind weed was winding in the other direction. (West of Denver) Was going to attempt a contact in the southern hemisphere but realize now that it would have to be the same bean seed. Ray Bisque From monique from mail.bio.tamu.edu Wed Jul 16 16:16:51 2008 From: monique from mail.bio.tamu.edu (monique) Date: Wed Jul 16 19:21:39 2008 Subject: [Plant-biology] Re: Direction of vine winding In-Reply-To: References: Message-ID: Ramon E. Bisque wrote: > Began searching when I noticed that my pole beans were all climbing > clockwise and a bind weed was winding in the other direction. (West of > Denver) Twining direction is determined by genus or species. Some plants are consistently clock-wise climbers and others are counter-clockwise. For fun, Google up the old Flanders and Swann song about the Honeysuckle... Monique Reed From jdemarie from verizon.net Thu Jul 24 15:49:39 2008 From: jdemarie from verizon.net (jdemarie@verizon.net) Date: Thu Jul 24 20:35:59 2008 Subject: [Plant-biology] Polonia seeds Message-ID: <16197180.4212841216932579140.JavaMail.root@vms069.mailsrvcs.net> If you are still interested, I have a mature tree near Buffalo, N.Y. and there was someone growing them in or near Cincinatti, Ohio. From rpalanivelu from hotmail.com Tue Jul 29 13:29:30 2008 From: rpalanivelu from hotmail.com (Ravi Palanivelu) Date: Tue Jul 29 23:22:03 2008 Subject: [Plant-biology] Early-bird registration reminder: Plant Reproductive biology conference in Tucson, AZ, USA Message-ID: Dear colleagues, We invite you and your lab to participate in an upcoming conference in the field of plant reproductive biology, "Frontiers in Sexual Plant Reproduction III" (http://www.ag.arizona.edu/FSPRIII), that will be held in Tucson, Arizona on October 17-19, 2008 in the Tucson Marriott University Park. Six major sessions have been planned for the conference: Male gametophyte development and function, Female gametophyte development and function, Endosperm development and imprinting, Compatible and incompatible pollen-pistil interactions, Evolution of plant reproduction, Systems biology of plant reproduction and emerging technologies Preliminary program and information on registration, abstracts, lodging and travel is posted at the FSPR III conference website: http://www.ag.arizona.edu/FSPRIII. **Early bird registration closes on July 31, 2008. Please register on or before this date to take advantage of low fees**. The registration fees for the three-day meeting includes food {Breakfast, 10/17- 10/19; lunch (10/17- 10/19) & dinner (10/17)}, transportation and visit to desert museum on 10/18 and banquet dinner in desert museum on 10/18. Participants are encouraged to submit an abstract and present a poster. Three faculty judges will identify top three posters for a cash award of $500 (first prize), $300 (second prize) and $200 (third prize). The prizes will be announced and awarded during the conference banquet dinner (October 18th, 2008). Please forward this to other colleagues who may be interested in this announcement. We look forward to seeing you in October 2008 for an exciting scientific meeting on plant reproduction. Sincerely, The organizers: Dr. Ravi Palanivelu, University of Arizona (rpalaniv@ag.arizona.edu) Dr. Dmitry Belostotsky, University of Missouri, Kansas City.