You have to use Reverse Transcriptase because it is the only enzyme to
synthesize cDNA from mRNA. If you want to amplify DNA from mRNA, you will use
two step reactions. First, you will get cDNA from reverstranscription using
RTase and then you can amplify numerous DNA molecules using PCR. Taq DNA
polymerase is used in this step.
From: Shammas <shammas at online.no>
Date: 1998? 6? 19? ??? PM 6:33
Subject: mRNA and Taq polymerase
>Why does Taq polymerase work only on cDNA and not mRNA?
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