In article <199507161426.WAA27105 at einstein.technet.sg>, pohyam at TECHNET.SG
>>I have always been using the two component OPD and BCIP/NBT coupled with
>for my ELISA assays. Recently, I read that a new single component TMB
>substrate for HRP is on sale. Has anyone tried this? Is it superior in
>of OD duplicity and stability of colour development? Can someone share
>experience with me?
We have used the single component TMB substrate for the past 2 years in
our single step ELISAs for drugs in the race horse. Use of the product
(K-Blue) has improved sensitivities, precision and made for easier
handling than the classical 2 component system.
K-Blue is available from ELISA TECHNOLOGIES in Lexington, KY